Kinetics of Thermal Destruction of Bacteriophages Active Against Streptococcus cremoris

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Abstract
The kinetics and thermodynamics of the heat inactivation for bacteriophages active against Streptococcus lactis C2 and C'10 were determined in skimmilk. Thermal destruction of Phage el0 at 55, 60, and 65 C and Phage c2 at 55 C followed the kinetics of a first-order reaction. However, at 60 and 65 C, the thermal destruction of Phage c2 failed to follow the kinetics of a first-order reaction. At 60.2 C, the rate of heat inactivation of Phage el0 and the second component of Phage c2 was the same. The D, k, and AF values for Phage c2 would indicate that it is more heat stable above 60.2 C and less heat stable below 60.2 C than c10 phage. Based on the high positive E a and AS values, it would appear that protein and nucleic acid denaturation is associated with the thermal inactivation process for Phage c10. In contrast, for Phage c2, low positive E a and negative AS values indicate that the thermal inactiva- tion of this phage at the temperatures studied involves a single point of attack of the deoxyribonucleic acid strand or a disorientation of a decisive protein.