In vitro Shoot Regeneration from Seedling Root Segments of Brassica oleracea and Brassica napus Cultivars

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Abstract
Root segments taken from aseptically-grown seedlings of Brassica oleracea var. italica cv. Green Comet were used in an investigation of factors affecting in vitro regeneration. Shoot regeneration was found to increase with seedling age and to be highest in root segments adjacent to the hypocotyl and lowest in segments adjacent to the root tip. In a comparison of a range of media and agar concentrations shoot formation was favoured by complex media containing reduced nitrogen and was higher on gelled media than in liquid medium. The effects of various cytokinins and auxins were investigated; KN was the best cytokinin and IAA and Picloram the best auxins for shoot induction. Root segments from six other Brassica cultivars were grown on the medium devised for Green Comet; shoots were regenerated from two B. oleracea cultivars and two B. napus cultivars, but not from the B. campestris cultivars tested.