Immunohistochemical Localization of Aromatase Cytochrome P-450 and Estradiol Dehydrogenase in the Syncytiotrophoblast of the Human Placenta*
- 1 October 1987
- journal article
- research article
- Published by The Endocrine Society in Journal of Clinical Endocrinology & Metabolism
- Vol. 65 (4) , 757-764
- https://doi.org/10.1210/jcem-65-4-757
Abstract
Immunohistochemistry employing immunoglobulin G fractions raised against aromatase cytochrome P-450 and antiserum against 17.beta.-estradiol dehydrogenase was used to localize these two steroid-coverting enzymes in the human placenta. Immunostaining for both enzymes was found exclusively in the syncytiotrophoblast, while the underlying cytotrophoblast and the villus core did not stain. Ultrastructural examination of aromatose cytochrome P-450- and 17.beta.-estradiol dehydrogenase-labeled sections disclosed immunoreactive product in the membranes of the endoplasmic reticulum; the nucleus, mitochondria, Golgi apparatus, and secretory granules were free of staining. These findings suggest that the syncytiotrophoblast is actively involved in the synthesis and metabolism of estrogens and in their role in placental endocrine function.This publication has 12 references indexed in Scilit:
- Immunocytochemical localization of aromatase in ovaries of some rodents, cow and human.ACTA HISTOCHEMICA ET CYTOCHEMICA, 1984
- Synaptology and sources of vasoactive intestinal polypeptide and substance p containing axons of the cat celiac ganglion. An experimental electron microscopic immunohistochemical studyNeuroscience, 1983
- Fine structure of serotonin-containing axons in the marginal zone of the rat spinal cordBrain Research, 1983
- A note on the use of picric acid-paraformaldehyde-glutaraldehyde fixative for correlated light and electron microscopic immunocytochemistryNeuroscience, 1982
- Use of avidin-biotin-peroxidase complex (ABC) in immunoperoxidase techniques: a comparison between ABC and unlabeled antibody (PAP) procedures.Journal of Histochemistry & Cytochemistry, 1981
- Apparent immunologic nonidentity of human placental and endometrial 17β-estradiol dehydrogenaseAmerican Journal of Obstetrics and Gynecology, 1980
- THE SUBCELLULAR DISTRIBUTION OF 17β-HYDROXYSTEROID DEHYDROGENASE IN THE HUMAN TERM PLACENTAActa Endocrinologica, 1976
- Biological Aromatization of SteroidsJournal of Biological Chemistry, 1959
- HUMAN PLACENTAL ESTRADIOL-17-BETA DEHYDROGENASE .1. CONCENTRATION, CHARACTERIZATION AND ASSAY1958
- THE INTERCONVERSION OF ESTRONE AND ESTRADIOL BY HUMAN TISSUE SLICES1,2,3Endocrinology, 1953