Effect of Sodium Chloride on the Activity and Production of Staphylococcal Exonuclease
- 1 January 1980
- journal article
- research article
- Published by Microbiology Society in Microbiology
- Vol. 116 (1) , 69-74
- https://doi.org/10.1099/00221287-116-1-69
Abstract
Summary: Extracellular nuclease activity in Staphylococcus aureus was enhanced about fourfold by 1% (w/v) NaCl, KCl, CsCl or LiCl. The pH and concentration of Ca2+ for optimum activity varied with the NaCl concentration; with an increased NaCl concentration, a higher Ca2+ concentration and a lower pH were required. Vmax, but not Kmax, varied with the concentration of NaCl. The addition of 3% (w/v) NaCl to growing cultures of S. aureus increased nuclease production fivefold.Keywords
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