Centrifugal Precipitation Chromatography − a Novel Chromatographic System for Fractionation of Polymeric Pigments from Black Tea and Red Wine

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Abstract
A novel chromatographic system was developed and first applied to the fractionation of polymeric pigments from black tea and red wine. Centrifugal precipitation chromatography (CPC) generates solvent gradients through a long separation channel under a centrifugal force field. Tea and wine extracts are precipitated in a hexane- or methyl tert-butyl ether-rich environment and are exposed to a gradually increasing ethanol concentration. This causes a repetitive precipitation and dissolution of the biopolymers along the channel. Consequently, they are eluted in the order of their solubility in the organic solvent. It is shown by HPLC analysis of the separated fractions that monomers elute first, whereas fractionated polymers can be found at the end of the chromatographic run. This novel method allows gentle fractionation of polymeric tea and wine constituents and also has potential for use in preparative-scale separations. Keywords: Centrifugal precipitation chromatography; high-speed countercurrent chromatography; black tea; red wine; fractionation; polymers; pigments; polyphenols; thearubigins