Amplification of the phosphorylation site ‐ ATP‐binding site cDNA fragment of the Na⁺,K⁺‐ATPase and the Ca²⁺‐ATPase of Drosophila melanogaster by polymerase chain reaction

4 December 1989

journal article
research article
Published by Wiley in FEBS Letters

Vol. 258 (2) , 203-207
https://doi.org/10.1016/0014-5793(89)81653-9

Abstract

In vitro DNA‐amplification technique has been utilized to generate a 430 bp fragment of the Na⁺,K⁺‐ATPase, and a 550 bp fragment of a Ca²⁺‐ATPase (the sarcoplasmic reticulum‐type) of Drosophila melanogaster. The oligonucleotide primers for the DNA‐amplification (Polymerase Chain Reaction) had been designed on the basis of amino acid sequence motifs ‐ the phosphorylation site and the ATP‐binding site ‐ conserved among members of the ATPase protein family. Using the amplified cDNA‐segments as probes, we demonstrated that there is one Na⁺,K⁺‐ATPase and one Ca²⁺‐ATPase (sarcoplasnaic reticulum‐type) gene in the Drosophila genome. Three different mRNA species are processed from the Na⁺,K⁺‐ATPase gene and one from the Ca²⁺‐ATPase gene. Developmental control in expression of the Ca²⁺‐ATPase gene was observed.

Keywords

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Amplification of the phosphorylation site ‐ ATP‐binding site cDNA fragment of the Na+,K+‐ATPase and the Ca2+‐ATPase of Drosophila melanogaster by polymerase chain reaction

Abstract

Keywords

Amplification of the phosphorylation site ‐ ATP‐binding site cDNA fragment of the Na⁺,K⁺‐ATPase and the Ca²⁺‐ATPase of Drosophila melanogaster by polymerase chain reaction