Autologous Skin Grafts in the Rat. Uptake of 35S-sulfate.

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Abstract
Summary Synthesis of sulfomucopolysaccharides, represented by uptake of 35S-sulfate into full-thickness skin autografts and skin of rats 48 hours after intraperitoneal injection of radioactively labeled sulfate was studied. Three and 5 days after surgery, the uptake into grafts was reduced as compared to that into symmetrical skin. This period was followed by an increased uptake into the grafts, demonstrable as long as 54 days after surgery. One hundred three days after operation, the uptake into the grafts was of the same order as that into symmetrical skin. The alterations in uptake of 35S-sulfate into the grafts are considered part of a connective-tissue response to injury. The uptake of 35S-sulfate into uninjured skin of operated rats was reduced as compared to that into skin of non-operated animals, except for skin of grafted animals 28 days after surgery. These findings are discussed in the light of a possible influence of stress and age upon the metabolic activity of sulfomucopolysaccharides.