Somatostatin Secretion from the Isolated Perfused Dog Ileum*

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Abstract
Ileal D cell function was examined. The influence of perfusate Ca, K, arginine and glucose on release of somatostatin (SS) was measured using an isolated, perfused dog ileum preparation. Ileal SS release was significantly enhanced by an increase in extracellular Ca from 0-1.3 mM (from 11 .+-. 3 pg/ml to 37 .+-. 11 pg/ml; 2 P < 0.05). In the presence of a normal Ca level (1.3 mM) an increase in K from 4.4-13.8 mM caused enhanced SS output (from 46 .+-. 8 pg/ml to 74 .+-. 10 pg/ml; 2 P < 0.05). The addition of arginine (10 mM) elicited a prominent rise in SS secretion (from 105 .+-. 8 pg/ml to 184 .+-. 30 pg/ml; 2 P < 0.05) while enhancement in glucose from 5-20 mM was without effect (57 .+-. 13 pg/ml vs. 57 .+-. 14 pg/ml). Apparently, ileal D cells release SS in response to changes in certain appropriate stimuli, but in contrast to those D cells situated in other parts of the gastrointestinal tract, e.g., the pancreas, they are apparently nonresponsive to glucose.