A Radioimmunological Assay Method for Insulin Using Insulin-125I and Gel Filtration
- 1 August 1965
- journal article
- research article
- Published by The Endocrine Society in Journal of Clinical Endocrinology & Metabolism
- Vol. 25 (8) , 1043-1049
- https://doi.org/10.1210/jcem-25-8-1043
Abstract
A radioimmunological assay for insulin is described using insulin-I125 as a tracer and gel filtration on Sephadex G75 to effect the separation of bound from free insulin. The relatively long half-life (60 days) and the absence of [beta] radiation by this isotope decreases the radiation hazard encountered during iodination procedures. As little as 0.25 [mu]U of insulin can be reliably measured by a single tracer for 6 months.Keywords
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