Single‐Molecule Quantum‐Dot Fluorescence Resonance Energy Transfer
- 22 April 2005
- journal article
- research article
- Published by Wiley in Chemphyschem
- Vol. 6 (5) , 956-960
- https://doi.org/10.1002/cphc.200400557
Abstract
Colloidal semiconductor quantum dots are promising for single-molecule biological imaging due to their outstanding brightness and photostability. As a proof of concept for single-molecule fluorescence resonance energy transfer (FRET) applications, we measured FRET between a single quantum dot and a single organic fluorophore Cy5. DNA Holliday junction dynamics measured with the quantum dot/Cy5 pair are identical to those obtained with the conventional Cy3/Cy5 pair, that is, conformational changes of individual molecules can be observed by using the quantum dot as the donor.Keywords
This publication has 41 references indexed in Scilit:
- Single-Molecule Three-Color FRETBiophysical Journal, 2004
- Exploring Rare Conformational Species and Ionic Effects in DNA Holliday Junctions Using Single-molecule SpectroscopyJournal of Molecular Biology, 2004
- Probing Single-Stranded DNA Conformational Flexibility Using Fluorescence SpectroscopyPublished by Elsevier ,2004
- Near-Complete Suppression of Quantum Dot Blinking in Ambient ConditionsJournal of the American Chemical Society, 2004
- Quantum-Dot-Functionalized Scanning Probes for Fluorescence-Energy-Transfer-Based MicroscopyThe Journal of Physical Chemistry B, 2003
- Diffusion Dynamics of Glycine Receptors Revealed by Single-Quantum Dot TrackingScience, 2003
- Structural dynamics of individual Holliday junctionsNature Structural & Molecular Biology, 2002
- Programmed Assembly of DNA Functionalized Quantum DotsJournal of the American Chemical Society, 1999
- MR Imaging Contrast Enhancement Based on Intermolecular Zero Quantum CoherencesScience, 1998
- Probing the interaction between two single molecules: fluorescence resonance energy transfer between a single donor and a single acceptor.Proceedings of the National Academy of Sciences, 1996