Calibration for Instrumental Spreading in Size Exclusion Chromato-Graphy by a Novel Recycle Technique
- 1 May 1986
- journal article
- research article
- Published by Taylor & Francis in Journal of Liquid Chromatography
- Vol. 9 (6) , 1141-1161
- https://doi.org/10.1080/01483918608075496
Abstract
So far, the best available techniques for the instrumental spreading calibration in size exclusion chromatography (SEC) provide the first moments of the spreading function, and require the measurement of several recycles. This work presents a method that enables the calculation of the spreading, making no “a priori” assumptions with regards to its shape, from the information contained in the original and first recycle chromatograms of a narrowly distributed standard only. Then, the corrected chromatogram may be obtained. The technique is tested on experimental data.This publication has 11 references indexed in Scilit:
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