Molecular changes associated with tuberisation in Solanum tuberosum. Differential expression of α‐tubulin isotypes
- 1 February 1991
- journal article
- Published by Wiley in Physiologia Plantarum
- Vol. 81 (2) , 244-250
- https://doi.org/10.1111/j.1399-3054.1991.tb02137.x
Abstract
Changes in gene expression that occur in the stolon tips of potato (Solanum tuberosum L.) cv. Record during tuberisation were investigated. Protein extracts from stolon tips at various stages in the tuberisation process were analysed by two‐dimensional gel electrophoresis. A number of quantitative and qualitative changes in polypeptide composition accompanied the very early stages of tuberisation. In vitro translation of RNA extracted from stolon tips also revealed quantitative and qualitative changes associated with tuberisation. Immunoblotting of protein extracts with monoclonal antibodies raised against α‐ and β‐tubulin showed quantitative changes in the relative level of β‐tubulin, but not α‐tubulin, as the stolon tips tuberised. Changes in the pattern of α‐tubulin isotype expression were shown to occur at early stages in the tuberisation process.Keywords
This publication has 26 references indexed in Scilit:
- Tubulin Isotypes in Rye Roots Are Altered during Cold AcclimationPlant Physiology, 1990
- The steady-state level of potato sucrose synthase mRN A is dependent on wounding, anaerobiosis and sucrose concentrationGene, 1989
- The nature of protein differentiation in the growing pea rootPlant, Cell & Environment, 1989
- The β-tubulin gene family of Arabidopsis thaliana: preferential accumulation of the β1 transcript in rootsGene, 1988
- Monoclonal antibody to intermediate filament antigen cross-reacts with higher plant cells.The Journal of cell biology, 1985
- Multiple isotypes of α‐ and β‐tubulin in the plant Phaseolus vulgarisFEBS Letters, 1985
- Molecular Biology and Genetics of TubulinAnnual Review of Biochemistry, 1985
- TECHNOLOGICAL EXAMINATION OF LOW‐FIRED TERRACOTTA STATUES FROM AYIA IRINI, KEAArchaeometry, 1982
- Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications.Proceedings of the National Academy of Sciences, 1979
- Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4Nature, 1970