A DEMONSTRATION OF THE NATURE OF POLYHEDRA USING ALKALINE SOLUTIONS

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Abstract
Polyhedral bodies from virus-diseased larvae of 2 insects, Prodenia praefica and Colias philodice eurytheme, were exposed to dilute NaOH for varying lengths of time and were examined with the electron microscope and dark-field microscope. These studies showed that polyhedral bodies are apparently made up of 3 components (1) an outer envelope, (2) a dense inner substance, and (3) bundles of virus particles contained in the inner substance. The alkaline soln. ordinarily dissolves only the inner substance leaving the envelope enclosing many virus bundles. With the dark-field microscope, the virus bundles appear to be identical with the bright granules reported by Paillot and others.
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