RADIOIMMUNOIMAGING IN MALIGNANT-MELANOMA WITH IN-111-LABELED MONOCLONAL-ANTIBODY 96.5

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  • 1 January 1985
    • journal article
    • research article
    • Vol. 45  (5) , 2376-2381
Abstract
A radiolabeled monoclonal antibody (96.5) reactive with an MW 97,000 antigen found on over 80% of melanoma cell lines and tissue extracts was examined for its ability to detect malignant melanoma metastases in vivo. For imaging purposes, it was conjugated with diethyltriaminepentaacetic acid and subsequently labeled with 111In by chelation. Patients (31) with metastatic melanoma received single injections of monoclonal antobody 96.5 at concentrations ranging form 0.5-20 mg and at specific activities of 111In ranging from 0.125-4 mCi/mg. Total-body scans were performed at various time intervals following administration. No serious side effects were observed. Of a total of 100 previously documented metastatic sites, 50 imaged for a specificity of 50%. The number of sites imaged increased significantly as the amount of antibody administered increased relative to the average radiation dose. Considerable background uptake of isotope was observed in blood pool and other organs with gradual acquisition of label in tumor sites by 48 to 72 h. Tumor imaging of melanoma using 111In-labeled monoclonal antibody 96.5 appeared feasible, especially at antibody doses above 2 mg.