A Histologic and Histochemical Study of Lipids in Human Odontoblasts

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Abstract
Human odonto-blasts were subjected to a variety of histochemical procedures to assist in the analysis of the intracellular granules which were demonstrated in the cytoplasm and odontoblastic process of cells fixed in Flemming''s strong solution. Sudan-B and Sudan-B pyridine extraction, Sudan-B in ethylene-glycol, lipid-crimson and the Lorraine-Smith-Dietrich stain gave positive results for lipid while the Maxillon-Blue RL for muco-polysaccharides was also positive. The Nile-Blue-Sulphate-Safranin method for phospholipids was erratic. The cytoplasmic granules in odontoblasts are composed of a complex consisting mostly of lipid and small amounts of mucopolysaccharides.