Ecdysteroid Metabolism by Soluble Enzymes from an Insect. Metabolic Relationships between 3β-Hydroxy-, 3α-Hydroxy- and 3-Oxoecdysteroids
- 1 January 1984
- journal article
- research article
- Published by Walter de Gruyter GmbH in Hoppe-Seyler´s Zeitschrift Für Physiologische Chemie
- Vol. 365 (2) , 809-818
- https://doi.org/10.1515/bchm2.1984.365.2.809
Abstract
The formation and fate of 3-dehydroecdysteroids were studied in vitro in tissue extracts from P. brassicae (Lepidoptera). Three cytosolic enzymes are involved: an ecdysone oxidase converts 3.beta.-hydroxyecdysteroids into 3-dehydroecdysteroids and 2 different reductases, requiring NADPH as cofactor, transform 3-dehydroecdysteroids into the 3.alpha.-hydroxy- or the 3.beta.-hydroxy forms, respectively. 3.alpha.-hydroxyecdysteroid formation is only detected in the gut; all tissues contain the 2 other activities. Conversion of 3.beta.- to 3.alpha.-hydroxyecdysteroids apparently proceeds through 3-dehydro compounds. The biological significance of these reactions is discussed.This publication has 14 references indexed in Scilit:
- Formation of imine derivatives between Tris(hydroxymethyl)-aminomethane and saturated 3-oxo steroids during conventional work up of biological samples dissolved in Tris-HCl buffersThe Journal of Steroid Biochemistry and Molecular Biology, 1983
- Conversion of ecdysone and 20‐Hydroxyecdysone into 26‐OIC derivatives is a major pathway in larvae and pupae of species from three insect ordersArchives of Insect Biochemistry and Physiology, 1983
- Sample processing for high-performance liquid chromatography of ecdysteroidsJournal of Chromatography A, 1982
- The relative biological activities of α- and β-ecdysone and their 3 dehydro derivatives in the chromosome puffing assayJournal of Insect Physiology, 1978
- Ecdysone Oxidase in InsectsHoppe-Seyler´s Zeitschrift Für Physiologische Chemie, 1978
- Catalytical oxidation of ecdysteroids to 3-dehydro products and their biological activitiesJournal of Insect Physiology, 1977
- Enzymatic and Chemical Synthesis of 3-Dehydroecdysterone, a Metabolite of the Moulting Hormone of InsectsHoppe-Seyler´s Zeitschrift Für Physiologische Chemie, 1977
- Ecdysone Oxidase, an Enzyme from the BlowflyCalliphora erythrocephala(Meigen)Hoppe-Seyler´s Zeitschrift Für Physiologische Chemie, 1975
- 3-Dehydroecdyson, ein Stoffwechselprodukt des Ecdysons bei der SchmeißfliegeCalliphora erythrocephalaMeigenHoppe-Seyler´s Zeitschrift Für Physiologische Chemie, 1972
- A Lepidopteran Saline: Effects of Inorganic Cation Concentrations On Sensory, Reflex and Motor Responses in A Herbivorous InsectJournal of Experimental Biology, 1966