Regulation of type V adenylyl cyclase by PMA‐sensitive and ‐insensitive protein kinase C isoenzymes in intact cells
- 22 April 1996
- journal article
- Published by Wiley in FEBS Letters
- Vol. 384 (3) , 273-276
- https://doi.org/10.1016/0014-5793(96)00331-6
Abstract
Type V adenylyl cyclase (AC) was stably over-expressed in HEK293 cells (293AC-V). Forskolin-stimulated cAMP accumulation in 293AC-V was 5 times as great as that in control cells. PMA, a protein kinase C (PKC) activator, enhanced cAMP accumulation in 293AC-V cells dose-and time-dependently and this enhancement was abolished by staurosporine. Insulin also enhanced cAMP accumulation in 293AC-V cells. Co-transfection of PKC-ζ, but not PKC-α, potentiated the effects of insulin. These data suggest that type V AC activity is regulated in cells by PKC isoenzymes through different extracellular stimuli.Keywords
This publication has 26 references indexed in Scilit:
- Insulin-induced Activation of Phosphatidylinositol (PI) 3-KinasePublished by Elsevier ,1995
- Expression of Type V Adenylyl Cyclase Is Required for Epidermal Growth Factor-mediated Stimulation of cAMP AccumulationPublished by Elsevier ,1995
- Localization of Protein Kinases by Anchoring Proteins: a Theme in Signal TransductionScience, 1995
- Protein kinase C - a question of specificityTrends in Biochemical Sciences, 1994
- Phorbol ester stimulation of the type I and type III adenylyl cyclases in whole cellsBiochemistry, 1993
- Epidermal Growth Factor Produces Inotropic and Chronotropic Effects in Rat Hearts by Increasing Cyclic AMP AccumulationGrowth Factors, 1993
- Intracellular Signaling by Hydrolysis of Phospholipids and Activation of Protein Kinase CScience, 1992
- ‘Crosstalk’: a pivotal role for protein kinase C in modulating relationships between signal transduction pathwaysEuropean Journal of Biochemistry, 1991
- Cross-talk between cellular signalling pathways suggested by phorbol-ester-induced adenylate cyclase phosphorylationNature, 1987
- Protein kinase C phosphorylates the inhibitory guanine‐nucleotide‐binding regulatory component and apparently suppresses its function in hormonal inhibition of adenylate cyclaseEuropean Journal of Biochemistry, 1985