Effects of 2‐methylisoborneol (MIB), and ethanol on the expression and activity of cytochrome P450s from the channel catfish

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Abstract
Injection of 1 mg kg−1 of 2‐methylisoborneol (MIB) caused an increase in a CYP1A‐like protein of approximately 56 kDa from the kidneys of juvenile channel catfish as determined by western blot analysis using antibodies raised against trout CYP1A1. Ethoxyresorufin O‐deethylase activity from kidneys of MIB‐injected fish was significantly elevated as compared to ethanol‐injected controls. Static exposure of juvenile channel catfish to 10 ppm MIB caused a statistically significant induction of a CYP2K‐like protein of approximately 53 kDa from the livers of treated catfish as determined by western blot analysis using antibodies raised against trout CYP2K1. Treatment of juvenile channel catfish with ethanol (1 ml kg−1) reduced the expression of one kidney and two constitutive liver P450s, while increasing another kidney form. There was no difference in carbon tetrachloride‐induced lipid peroxidation in livers after ethanol treatment. Thus, MIB and ethanol affect the expression of at least three P450 isoforms in channel catfish tissues.

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