Monkey Glutathione S-Aryltransferases

Publisher Website
Google Scholar
Abstract
The distribution of activity of glutathione S-aryltransferases [EC 2.5.1.13] in various tissues of Japanese monkey (Macaca fuscata) was examined using 1,2-dichloro-4-nitrobenzene and glutathione as substrates. The activity in liver was highest; it was roughly or over 10 times higher than those of other tissues. In addition, the enzyme activity was found to be distributed fairly evenly in various regions of the brain of crab-eating monkey (Macaca fascicularis). The enzymes were purified from the liver of rhesus monkey (Macaca mulatta) on a preparative scale by a combination of column chromatographies on Sephadex G-l00, CM-cellulose, and hydroxylapatite. Upon chromatography on CM-cellulose, five enzyme fractions were obtained and the main enzyme fraction was purified to apparent homogeneity as determined by polyacrylamide disc gel electrophoresis. The enzymes were also purified on a smaller scale from the liver of Japanese monkey. Upon chromatography on CM-cellulose, similar enzyme fractions were obtained and the major one was further purified to apparent homogeneity. The substrate specificity of the major enzyme purified from rhesus monkey liver was examined using various compounds and compared with those of the other four fractions partially purified by CM-cellulose chromatography. The action spectra of these enzyme fractions toward several substrates were all roughly similar.

This publication has 5 references indexed in Scilit: