EFFECT OF CELL-DENSITY AND CONFLUENCY ON CHOLESTEROL-METABOLISM IN CANCER-CELLS IN MONOLAYER-CULTURE

  • 1 January 1981
    • journal article
    • research article
    • Vol. 41  (2) , 473-477
Abstract
Cholesterol metabolism in 4 [human] gynecological cancer cell lines in monolayer culture was evaluated as a function of cell density. The rate of uptake and degradation of [125I]iodinated low-density lipoprotein increased during the 1st 24-48 h of culture but decreased thereafter. Once the cells became confluent, the rate of metabolism of [125I]iodinated low-density lipoprotein was only one-tenth that in cells which were in the preconfluent state. The specific activity of 3-hydroxy-3-methylglutaryl coenzyme reductase increased during the 1st 24-48 h of culture and subsequently declined, reaching a nadir after confluency was attained. The rate of incorporation of [14C]oleate into cholesteryl esters was low when the cells were in the log-exponential phase of replication but increased gradually as cell density increased. The highest specific activity of acylcoenzyme A: cholesterol acyltransferase was attained after the cells became confluent. Generally speaking, there was an inverse relationship between the specific activity of 3-hydroxy-3-methylglutaryl coenzyme A reductase and the rate of [125I]iodinated low-density lipoprotein metabolism and cholesteryl ester synthesis. Cholesterol metabolism in cancer cells in monolayer culture is regulated, in part, by the rate of cell division. Cholesterol metabolism was subject to the same regulatory mechanisms as are present in nonneoplastic cells.

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