Linkage of Ribosomal RNA Genes in Leptospira

Publisher Website
Google Scholar
Abstract
We determined the linkage of 16S, 23S, and 5S rRNA genes in several strains of Leptospira and Leptonema by DNA-DNA hybridization. Almost all the hybridizations in all leptospires used in these experiments gave two radioactive bands and the results strongly suggest that the number of the 16S and the 23S rRNA genes in those strains is two, respecitvely. In contrast with the larger rRNAs, the number of 5S rRNA gene was different. In the strains of leptospires, Leptospira biflexa, which were non-parasitic, there are two genes for 5S rRNA, whereas only one gene for 5S rRNA is carried in Leptospira interrogans, which were originally isolated as parasitic. Southern hybridization experiments suggest that those rRNA genes are interpersed on the leptospiral chromosome.