The protein kinase C activator phorbol‐12‐myristate‐13‐acetate enhances cyclic AMP accumulation in pheochromocytoma cells
- 3 February 1986
- journal article
- Published by Wiley in FEBS Letters
- Vol. 196 (1) , 131-134
- https://doi.org/10.1016/0014-5793(86)80227-7
Abstract
The protein kinase C activator, phorbol-12-myristate-13-acetate (PMA), augments the cyclic AMP accumulation induced by forskolin in pheochromocytoma (PC 12) cells with an EC50 value of 14 nM, while having no effect on basal values. At a concentration of 100 nM PMA markedly augmented the magnitude of the forskolin response and, in addition, caused a slight increase in the potency of forskolin. PMA also enhanced the maximal cyclic AMP accumulation produced by 2-chloroadenosine, and caused a slight increase in potency of the adenosine analog. Since PMA mimics the effect of diacylglycerols that form during the turnover of the membrane lipid, phosphatidylinositol, the results suggest an interrelationship between the systems involved in phosphatidylinositol turnover and cyclic AMP generation in PC 12 cells.Keywords
This publication has 19 references indexed in Scilit:
- Angiotensin II and phorbol ester enhance isoproterenol- and vasoactive intestinal peptide (VIP)-induced cyclic AMP accumulation in vascular smooth muscle cellsBiochemical and Biophysical Research Communications, 1985
- Sensitization of frog erythrocyte adenylate cyclase system by tumor-promoting phorbol diestersCanadian Journal of Physiology and Pharmacology, 1985
- Modulation of cyclic AMP accumulation in GH3 cells by a phorbol ester and thyroliberinBiochemical and Biophysical Research Communications, 1985
- An activator of protein kinase C (phorbol‐12‐myristate‐13‐acetate) augments 2‐chloroadenosine‐elicited accumulation of cyclic AMP in guinea pig cerebral cortical particulate preparationsFEBS Letters, 1985
- Muscarinic receptor-induced phosphoinositide hydrolysis at resting cytosolic Ca2+ concentration in PC12 cells.The Journal of cell biology, 1985
- Activation of protein kinase C potentiates isoprenaline-induced cyclic AMP accumulation in rat pinealocytesNature, 1985
- The Action of Adenosine Analogs on PC12 CellsJournal of Neurochemistry, 1981
- Tumour promoter uncouples β-adrenergic receptor from adenyl cyclase in mouse epidermisNature, 1980
- In vitro studies on the mode of action of the phorbol esters, potent tumor promoters: Part 1CRC Critical Reviews in Toxicology, 1980
- Nerve growth factor induced turnover of phosphatidylinositol in rat superior cervical gangliaBiochemical and Biophysical Research Communications, 1978