Improved processes for the production and isolation of dynemicin A and large-scale fermentation in a 10000-liter fermentor
- 1 November 1992
- journal article
- Published by Oxford University Press (OUP) in Journal of Industrial Microbiology & Biotechnology
- Vol. 11 (1) , 7-12
- https://doi.org/10.1007/bf01583725
Abstract
Supplementing the culture ofMicromonospora chersina sp. nov. No. M956-1 with NaI (0.5 mg/l) enhanced the production of dynemicin A by 35-fold in shake flask culture. Homogeneous dynemicin A was obtained from the whole broth extract by Dicalite chromatography, Sephadex LH-20 chromatography and vacuum liquid chromatography. Gram quantities of dynemicin A were obtained from the fermentation ofM. chersina sp. nov. No. M956-1 in a 10000-liter fermentor.Keywords
This publication has 12 references indexed in Scilit:
- DNA intercalation and cleavage of an antitumor antibiotic dynemicin that contains anthracycline and enediyne cores.Proceedings of the National Academy of Sciences, 1990
- Crystal and molecular structure of dynemicin A: a novel 1,5-diyn-3-ene antitumor antibioticJournal of the American Chemical Society, 1990
- New antibiotic produced by Micromonospora globosa.The Journal of Antibiotics, 1990
- Nucleotide-specific cleavage and minor-groove interaction of DNA with esperamicin antitumor antibiotics.Proceedings of the National Academy of Sciences, 1989
- Esperamicins, a class of potent antitumor antibiotics: mechanism of action.Proceedings of the National Academy of Sciences, 1989
- Calicheamicins, a novel family of antitumor antibiotics. Taxonomy, fermentation and biological properties.The Journal of Antibiotics, 1989
- Dynemicin A, a novel antibiotic with the anthraquinone and 1,5-diyn-3-ene subunit.The Journal of Antibiotics, 1989
- Calichemicins, a novel family of antitumor antibiotics. 2. Chemistry and structure of calichemicin .gamma.1IJournal of the American Chemical Society, 1987
- Esperamicins, a novel class of potent antitumor antibiotics. 3. Structures of esperamicins A1, A2, and A1bJournal of the American Chemical Society, 1987
- Esperamicins, a novel class of potent antitumor antibiotics. I. Physico-chemical data and partial structure.The Journal of Antibiotics, 1985