Measurement of Active Renin by the 4G1 Anti-Human Renin Monoclonal Antibody

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Abstract
Three monoclonal human renin antibodies have been selected to settle two immunoradiometric assays of human plasma rénin (IRMA). The first pair of antibodies 3E8-4G1* recognizes exclusively active renin (AR), as demonstrated by the lack of increase either of the number of AR molecules or the renin enzymatic activity, when plasma is set free of inactive renin (IR) by immunoaffinity chromatography with human renin prosegment monoclonal antibody. The second pair of monoclonal antibodies 4E1-3E8* gives results which are very significantly correlated to those obtained with the 3E8-4G1* pair after trypsin activation. The standardization of renin measurements with use of monoclonal antibodies, and the standardization of renin will allow the exchange of comparable informations between the various laboratories involved in the investigation of the renin-angiotensin system in humans.