Gas-Liquid Chromatography of Free Fatty Acids

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Abstract
Fatty acids may be separated by gas-liquid chromatography (GLC) as the free acids if appropriate packings are used. This was first recognized by James and Martin1, who studied the separation of C1−C12 acids and found it necessary to add organic or inorganic acids to the liquid phase to reduce tailing of the peaks. Subsequent work has confirmed the correctness of these measures and has led to the preparation of improved columns for an effective separation of both saturated and unsaturated fatty acids of short and long chain length in the free form. The latter developments have been fostered by the requirement for a rapid and direct determination of individual free fatty acid levels in extracts of clinical, biochemical and industrial sources.