Multiplication ofPseudomonas syringaepv.glycineaon Soybean Primary Leaves Exposed to Aerosolized Inoculum

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Abstract
Aerosol generated from a suspension containing 2 .times. 108 colony-forming units (CFU) of P. syringae var. glycinea/m was admitted into a stirred-settling chamber containing potted ''Acme'' soybean plants with unifoliolate leaves fully unfolded and 1/2-2/3 expanded. Epiphytic populations averaging 103 and 104 CFU/cm-2 developed in 9 and 14 days, respectively, on leaves that were wet gently with atomized water before inoculation and kept at 20.degree. C without further wetting. Infection did not take place on these leaves. Bacterial blight symptoms developed consistently on leaves that were either wounded and wet or water-soaked and wetted immediately before inoculation. Interposition of up to 48 h between water-soaking and inoculation did not prevent infection if leaves were wetted again immediately before exposure to aerosol. Bacteria did not become established upon or within untreated leaves.