Immunodiagnosis of human dirofilariasis by enzyme-linked immunosorbent assay using recombinant DNA-derived fusion protein
- 1 September 1992
- journal article
- Published by Cambridge University Press (CUP) in Journal of Helminthology
- Vol. 66 (3) , 220-226
- https://doi.org/10.1017/s0022149x00014590
Abstract
An enzyme-linked immunosorbent assay (ELISA) using antigenic β-galactosidase-Dirofilaria immitis recombinant fusion protein (FP) obtained by the recombinant DNA technique provided a useful diagnostic tool for human dirofilariasis. D. immitis-infected human sera reacted strongly with FP that was immobilized with anti-β-galactosidase monoclonal antibody on microplates. However, the FP did not react with sera from patients with other filariasis. In detection of anti-D. immitis IgG antibody. ELISA using FP was highly sensitive and specific compared to that using crude somatic antigen.Keywords
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