Substrate channeling: alpha-ketobutyrate inhibition of acetohydroxy acid synthase in Salmonella typhimurium
- 1 September 1980
- journal article
- research article
- Published by American Society for Microbiology in Journal of Bacteriology
- Vol. 143 (3) , 1509-1512
- https://doi.org/10.1128/jb.143.3.1509-1512.1980
Abstract
Excess alpha-ketobutyrate inhibited the growth of Salmonella typhimurium LT2 by inhibiting the acetohydroxy acid synthase-catalyzed synthesis of alpha-acetolactate (a valine precursor). As a result, cells were starved for valine, and both ilvB (encoding acetohydroxy acid synthase I) and ilvGEDA (ilvG encodes acetohydroxy acid synthase II) were derepressed. The addition of valine reversed the effects of alpha-ketobutyrate.This publication has 13 references indexed in Scilit:
- Improved synthesis of the sodium salt of dl-α,β-dihydroxyisovaleric acidAnalytical Biochemistry, 1980
- PHYSIOLOGICAL CHARACTERIZATION OF POLAR Tn5-INDUCED ISOLEUCINE-VALINE AUXOTROPHS IN ESCHERICHIA COLI K-12: EVIDENCE FOR AN INTERNAL PROMOTER IN THE ilvOGEDA OPERONGenetics, 1979
- Mutations affecting the formation of acetohydroxy acid synthase II in Escherichia coli K-12Molecular Genetics and Genomics, 1979
- A comparative study of the acetohydroxy acid synthase isoenzymes of Escherichia coli K-12Biochimica et Biophysica Acta (BBA) - General Subjects, 1978
- Regulation of synthesis of the acetohydroxy acid synthase I isoenzyme in Escherichia coli K-12Biochimica et Biophysica Acta (BBA) - General Subjects, 1978
- Mutagenesis by insertion of a drug-resistance element carrying an inverted repetitionJournal of Molecular Biology, 1975
- Isoleucine and valine metabolism in Escherichia coli. XX. Multiple forms of acetohydroxy acid synthetaseBiochemical and Biophysical Research Communications, 1972
- Two forms of biosynthetic acetohydroxy acid synthetase in SalmonellatyphimuriumBiochemical and Biophysical Research Communications, 1972
- ISOLEUCINE AND VALINE METABOLISM IN ESCHERICHIA COLI .10. ENZYMATIC FORMATION OF ACETOHYDROXYBUTYRATE1961
- A COLORIMETRIC DETERMINATION OF BLOOD ACETOINJournal of Biological Chemistry, 1945