Determination of L-Glutamic Acid by Use of L-Glutamic Acid Decarboxy-lase from Mycobacterium phlei

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Abstract
A cell-free preparation from M. phlei with decarboxylase activity towards L-glutamic acid only is described. The preparation is inactive towards 18 other amino acids tested; no glutaminase or glutamoracemase activities were detected. Direct estimation of L-glutamic acid in natural products (containing glutamine) is thus possible. The enzyme may be preserved in solution at 0[degree]C for several months or lyophilized without loss of activity. Optimal decarboxylase activity lies between pH 5.2-5.4, falling off rapidly at pH values of 4 and 6,respectively. Some purification of the enzyme was achieved by fractionation with ammonium sulfate.

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