Further Observations on the Inhibitory Action of Ammonium Chloride on Influenza Virus.

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Abstract
The effect of NH4CI on viral penetration was measured in [chick embryo] chorioallantoic tissue in vitro by neutralizing absorbed virus with antiserum and determining the subsequent 24 hr. yield of hemagglutinins. In some experiments conditions for single cycle replication were obtained by leaving the antibody in the supernatant during the entire period of growth and then measuring cell-associated hemagglutinins. Although some reduction of the amount of penetrated virus was demonstrable 1 hr. after adding virus and NH4CI this later became insignificant. The prevention of viral penetration by NH4CI was considered too inefficient and irregular to account for the over-all inhibitory effect of NH4CI on viral replication. Inhibition by NH4CI of a type B (Lee) strain was comparable with that for Type A and reversibility of inhibition was demonstrated.