Radiation-Induced DNA Damage and Cellular Lethality in Cultured Mammalian Cells
- 1 June 1984
- journal article
- research article
- Published by JSTOR in Radiation Research
- Vol. 98 (3) , 479-490
- https://doi.org/10.2307/3576482
Abstract
X-ray induced DNA scissions and their repair were studied by an alkaline separation method. DNA damage in Chinese hamster [lung] V79 cells was assigned to 1 of 3 groups based on a repair profile previously used for mouse [lymphocytic leukemia] L5178Y cells: fast-reparable (t1/2 [half time] = 5 min), slow-reparable (t1/2 = 70 min) and nonreparable. The 3 kinds of damage were investigated in relation to cellular lethality under conditions where radiosensitivity of cultured cells was modified: different sensitivity in different cell lines, cell cycle fluctuation of radiosensitivity and recovery after split-dose irradiation. Among the 3 types of lesions, only nonreparable damage or remaining lesions showed correlation to cell killing. The parallel relationship between nonreparable damage and cell killing implies that this type of damage could play an important role in radiation-induced cell death.This publication has 17 references indexed in Scilit:
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