Survival of Bovine Spermatozoa Stored at 5 and 25° C. In Extenders Containing Varying Levels of Egg Yolk, Glucose, Glycine, Glycerol, Citrate, and Other Salts

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Abstract
The addition of 10% glycerol improved survival of sperm in a 20% egg yolk-glycine medium, but not in 50% egg yolk-glycine. Survival time of the spermatozoa was increased by storing them in a 20% egg yolk extender containing 1.16% sodium citrate dihydrate, 0.75% glycine, 1.0% glucose, 0.3% sulfanilamide (CU-16), and in a more complicated extender containing in addition sodium bicarbonate, potassium chloride and citric acid (CUE). In 2 experiments the percentage of motile spermatozoa over a 12-day storage period at 5[degree]C in CU-16 averaged 49 and 52, and in CUE averaged 50 and 52 (P > 0.05). The high sperm survival in both of these extenders at 5[degree]C indicated they might be useful in preserving the fertility of sperm stored for several days at this temperature.