Diluters for Bovine Semen. IX. Motility of Bovine Spermatozoa in Milk-Glycine and Egg Yolk–Glycine Diluents with and without Glycerol

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Abstract
A series of laboratory experiments was conducted in which livability of bovine spermatozoa was compared in combinations of aqueous solutions of glycine with (a) fresh skimmilk, (b) reconstituted nonfat dry milk solids (NFDMS), or (c) egg yolk. The effect of the addition of glycerol to these dilutents was also studied. Each of 5 aqueous solutions of glycine (0.0625-1.0 [image]) was mixed with an equal volume of reconstituted NFDMS and the mixtures were tested as semen diluents. Based on this trial, the 0.5 [image]_ glycine solution was used in subsequent experiments. Fresh skimmilk (heated)-0.5 [image] glycine (1:1) sustained livability equally as well as did egg yolk-0.5 [image]_glycine (1:1), and significantly better than did heated skimmilk alone. Mean livability over a 20-day storage period at 5[degree]C in reconstituted NFDMS-0.5 [image] glycine was superior to that in reconstituted NFDMS alone, and the addition of glycerol or fructose to the NFDMS-0.5 [image] glycine resulted in a further improvement in livability.