Murine T cell-specific Ia antigens: monoclonal antibodies define an I-A-encoded T lymphocyte structure.

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Abstract
T lymphocytes express a unique I-A subregion-controlled surface molecule that is not expressed on B lymphocytes. Antisera and monoclonal antibodies recognizing this structure were produced. Exhaustive B cell adsorption of (A.TH .times. B10.HTT)F1 antiserum, produced against activated A.TL T cells, left antibodies that bind an I-Ak specificity on some B10.A(4R) T lymphocytes (11 .+-. 2%, mean .+-. SEM). Exhaustive B cell adsorption of (A/J .times. B10.MBR)F1 antiserum, produced against activated B10.A(5R) cells, left antibodies specific for an I-Ab determinant on B10.A(5R) T cells (17 .+-. 2%). A.TL-immune (A.TH .times. B10.HTT)F1 were fused with NS-1 myeloma cells and antibody-producing hybrid cells were identified by a fluorescent enzyme-linked immunosorbent microassay. Eight monoclonal antibodies were selected; these lyse 7-26% of peripheral T cells from I-Ak strain. Thymocytes and bone marrow cells do not express the I-Ak T cell determinant. Exhaustive B cell adsorption did not remove I-Ak T cell-specific monoclonal antibodies.