Measurement of IgG in equine blood by immunoturbidimetry and latex agglutination
- 1 March 1985
- journal article
- research article
- Published by Wiley in Equine Veterinary Journal
- Vol. 17 (2) , 125-129
- https://doi.org/10.1111/j.2042-3306.1985.tb02065.x
Abstract
Summary: This paper describes the quantitative measurement of IgG concentrations in equine blood/serum by turbidimetric analysis and the qualitative assessment using latex agglutination. The concentration of IgG in foal serum measured by turbidimetry correlated well with the results obtained by radial immunodiffusion (r = 0.91) and the γ‐globulins estimated from the electrophoresis of serum on cellulose acetate (r = 0.92). The method, using antibody‐coated latex beads, to detect foals with serum IgG concentrations of less than 4 g/litre (whole blood less than 2 g/litre) proved to be accurate in 96 per cent of cases and provided a quick and simple qualitative test, suitable for use outside the laboratory.This publication has 16 references indexed in Scilit:
- Light-Scattering Immunoassay of Specific Proteins: A ReviewAnnals of Clinical Biochemistry: International Journal of Laboratory Medicine, 1983
- An assessment of wastage in Thoroughbred racing from conception to 4 years of ageEquine Veterinary Journal, 1982
- Quantitation of Serum ImmunoglobulinsCRC Critical Reviews in Clinical Laboratory Sciences, 1982
- A RAPID, SPECIFIC TEST FOR DETECTING ABSORPTION OF COLOSTRAL IgG BY THE NEONATAL FOALAustralian Veterinary Journal, 1980
- HYPOGAMMAGLOBULINAEMIA IN FOALS: PREVALENCE ON VICTORIAN STUDS AND SIMPLE METHODS FOR DETECTION AND CORRECTION IN THE FIELDAustralian Veterinary Journal, 1980
- Immobilization of immunoglobulins on polystyrene latex beads: Characterization by density gradient centrifugationBiotechnology & Bioengineering, 1978
- Something Old, Something New, Something Borrowed, Something for You!Journal of Physical Education and Recreation, 1978
- Some Practical Aspects of the Transfer of Passive Immunity to Newborn FoalsEquine Veterinary Journal, 1974
- Studies on passive immunity in the foalJournal of Comparative Pathology, 1974
- Quantitative estimation of proteins by electrophoresis in agarose gel containing antibodiesAnalytical Biochemistry, 1966