C3-REACTED SEPHAROSE - PREPARATIVE METHOD FOR SEPARATING T AND B LYMPHOCYTES
- 1 January 1977
- journal article
- research article
- Vol. 27 (1) , 38-42
Abstract
Lymphocytes [human] bearing complement (C) receptors (CR+) were separated from lymphocyte suspensions using C3[the 3rd C component]-reacted Sepharose columns as solid phase immunoabsorbents. The retained CR+ lymphocytes were recovered by elution of the column with IgG [immunoglobulin G] fraction purified from a rabbit antiserum to human C3. This paper describes the method, its specificity and the yields of cells obtained.This publication has 13 references indexed in Scilit:
- Specificity of human lymphocyte complement receptors.The Journal of Experimental Medicine, 1975
- Human peripheral lymphocytes bearing both B-cell complement receptors and T-cell characteristics for sheep erythrocytes detected by a mixed rosette method.1974
- Inhibition by C3 fragments of C3-dependent rosette formation and antigen-induced lymphocyte transformation.1974
- BINDING OF SOLUBLE IMMUNE COMPLEXES TO HUMAN LYMPHOBLASTOID CELLSThe Journal of Experimental Medicine, 1974
- Combined Detection of Human T and B Lymphocytes by Rosette Formation with Sheep Erythrocytes and Zymosan-C3 ComplexesThe Journal of Immunology, 1974
- Cellular Immunoadsorbents: a Simplified Technique for Separation of Lymphoid Cell PopulationsThe Journal of Immunology, 1974
- Fate of HL-A antigens and antibodies at the lymphocyte surface.1973
- Specific Purification of Lymphocyte Populations on a Digestible ImmunoabsorbentThe Journal of Immunology, 1973
- SURFACE MARKERS ON HUMAN T AND B LYMPHOCYTESThe Journal of Experimental Medicine, 1972
- The dynamic state of the lymphocyte membrane. Factors affecting the distribution and turnover of surface immunoglobulinsEuropean Journal of Immunology, 1972