Serum Folic Acid Activity by Improved L. casei Assay.

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Abstract
An improved (Lactobacillus casei ATCC 7469 assay procedure for serum folic acid activity is described. Its range of usefulness over previous procedures is increased because of greater sensitivity, better accuracy in the lower ranges, stability of test organism and micro method applicability. With this improved assay, the overlap of serum folic acid activity levels between normal subjects and folic acid deficient patients was so great as to seriously question the utility of this determination as an index of folic acid deficiency.