Specific immunoassay of alpha-amylase isoenzymes in human serum.
Open Access
- 1 August 1985
- journal article
- research article
- Published by Oxford University Press (OUP) in Clinical Chemistry
- Vol. 31 (8) , 1331-1334
- https://doi.org/10.1093/clinchem/31.8.1331
Abstract
A monoclonal antibody (66C7) was prepared that specifically binds human salivary amylase (EC 3.2.1.1); it cross reacts with human pancreatic amylase by less than 1%. Two procedures are described for determination of isoamylases in human serum with this antibody: an enzyme immunoassay for determining amylase of salivary origin, and a routine method in which this amylase is immunoprecipitated and the remaining (pancreatic) amylase activity is assayed. Results by the two methods correlate well.This publication has 6 references indexed in Scilit:
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