Cloning sequencing of Lol pI, the major allergenic protein of rye‐grass pollen
- 25 February 1991
- journal article
- Published by Wiley in FEBS Letters
- Vol. 279 (2) , 210-215
- https://doi.org/10.1016/0014-5793(91)80151-r
Abstract
We have isolated a full length cDNA clone encoding the major glycoprotein allergen Lol pI. The clone was selected using a combination of immunological screening of a cDNA expression library and PCR amplification of Lol pI-specific transcripts. Lol pI expressed in bacteria as a fusion protein shows recognition by specific IgE antibodies present in sera of grass pollen-allergic subjects. Northern analysis has shown that the Lol pI transcripts are expressed only in pollen of rye-grass. Molecular cloning of Lol pI provides a molecular genetic approach to study the structure-function relationship of allergens.Keywords
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