Distinct roles of PP1 and PP2A-like phosphatases in control of microtubule dynamics during mitosis

Open Access
Publisher Website
Google Scholar
Abstract
Assembly of a mitotic spindle requires the accurate regulation of microtubule dynamics which is accomplished, at least in part, by phosphorylation–dephosphorylation reactions. Here we have investigated the role of serine‐threonine phosphatases in the control of microtubule dynamics using specific inhibitors in Xenopus egg extracts. Type 2A phosphatases are required to maintain the short steady‐state length of microtubules in mitosis by regulating the level of microtubule catastrophes, in part by controlling the the microtubule‐destabilizing activity and phosphorylation of Op18/stathmin. Type 1 phosphatases are only required for control of microtubule dynamics during the transitions into and out of mitosis. Thus, although both type 2A and type 1 phosphatases are involved in the regulation of microtubule dynamics, they have distinct, non‐overlapping roles.