Expression of human α1‐antitrypsin in Escherichia coli
- 23 January 1984
- journal article
- Published by Wiley in FEBS Letters
- Vol. 166 (1) , 67-70
- https://doi.org/10.1016/0014-5793(84)80046-0
Abstract
Complementary DNA coding for human α1‐antitrypsin has been placed under the control of the λPR promotor carrier by the expression vector pCQV2 [1]. In conditions which allow transcription from this promotor (thermoinactivation of the repressor), Escherichia coli cells harbouring the recombinant plasmid pULB1114 express human α1‐antitrypsin (± 9000 molecules/cell). The product has a M r of 44 000, corresponding to mature unglycosylated α1‐antitrypsin.Keywords
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