Rapid Identification ofXanthomonas campestrispv.campestrisby ELISA

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Abstract
A double-antibody sandwich (DAS)-ELISA was developed to detect Xanthomonas campestris pv. campestris in leaf disks sampled from cabbage in the field. Assay time was reduced from 3-5 days with semiselective media to 5 hr with DAS-ELISA. Sheep and rabbit antisera were used as primary and secondary antibodies. Goat antirabbit peroxidase and purified 5-aminosalicylic acid were used as the enzyme indicator system, measured at 450 nm. On the basis of 510 samples with a disease incidence of 56%, 96.8% of known positives were detected by DAS-ELISA as confirmed by isolation and pathogenicity tests. DAS-ELISA permitted the processing of large numbers of samples and facilitated identification of the pathogen in seedbeds, on weeds, and on cabbage plants that showed unusual symptoms.