DEACYLATED TRNA Phe BINDING TO A RETICULOCYTE RIBOSOMAL SITE FOR THE INITIATION OF POLYPHENYLALANINE SYNTHESIS
- 1 August 1969
- journal article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 63 (4) , 1431-1438
- https://doi.org/10.1073/pnas.63.4.1431
Abstract
The initiation of polyphenylalanine synthesis at low MgCl(2) concentration in the reticulocyte transfer system has been found to have a stringent requirement for deacylated tRNA(Phe). An initial poly-U-directed complex between deacylated tRNA(Phe) and ribosomes is formed. The onset of polyphenylalanine synthesis causes the rapid release of tRNA(Phe) from the complex. Thermal dissociation studies indicate that deacylated tRNA(Phe) and phenylalanyl-tRNA are bound to the same ribosomes. A total of three ribosomal tRNA binding sites is indicated.Keywords
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