Crystallization and preliminary X-ray crystallographic analysis of two vascular apoptosis-inducing proteins (VAPs) fromCrotalus atroxvenom
- 26 June 2006
- journal article
- Published by International Union of Crystallography (IUCr) in Acta Crystallographica Section F Structural Biology and Crystallization Communications
- Vol. 62 (7) , 688-691
- https://doi.org/10.1107/s1744309106022548
Abstract
VAPs are haemorrhagic snake-venom toxins belonging to the reprolysin family of zinc metalloproteinases. In vitro, VAPs induce apoptosis specifically in cultured vascular endothelial cells. VAPs have a modular structure that bears structural homology to mammalian ADAMs (a disintegrin and metalloproteinases). VAP1 is a homodimer with a MW of 110 kDa in which the monomers are connected by a single disulfide bridge. VAP2 is homologous to VAP1 and exists as a monomer with a MW of 55 kDa. In the current study, several crystal forms of VAP1 and VAP2 were obtained using the vapour-diffusion method and diffraction data sets were collected using SPring-8 beamlines. The best crystals of VAP1 and VAP2 generated data sets to 2.5 and 2.15 angstroms resolution, respectively.Keywords
This publication has 18 references indexed in Scilit:
- A novel metalloprotease from Vipera lebetina venom induces human endothelial cell apoptosisToxicon, 2005
- ADAMs: key components in EGFR signalling and developmentNature Reviews Molecular Cell Biology, 2005
- Therapeutic Benefits from Targeting of ADAM Family MembersBiochemistry, 2004
- A Novel Metalloprotease from Gloydius halys Venom Induces Endothelial Cell Apoptosis through Its Protease and Disintegrin-Like DomainsThe Journal of Biochemistry, 2003
- ADAMs: modulators of cell–cell and cell–matrix interactionsCurrent Opinion in Cell Biology, 2003
- The ADAMs family of proteins: from basic studies to potential clinical applicationsThrombosis and Haemostasis, 2003
- Purification, cDNA cloning and characterization of the vascular apoptosis‐inducing protein, HV1, from Trimeresurus flavoviridisEuropean Journal of Biochemistry, 2001
- cDNA Cloning and Characterization of Vascular Apoptosis-Inducing Protein 1Biochemical and Biophysical Research Communications, 2000
- Purification of a Vascular Apoptosis-Inducing Factor from Hemorrhagic Snake VenomBiochemical and Biophysical Research Communications, 1997
- Astacins, serralysins, snake venom and matrix metalloproteinases exhibit identical zinc‐binding environments (HEXXHXXGXXH and Met‐turn) and topologies and should be grouped into a common family, the ‘metzincins’FEBS Letters, 1993