Purification and Characterization of Milk Lipase. I. Purification
Open Access
- 1 April 1963
- journal article
- research article
- Published by American Dairy Science Association in Journal of Dairy Science
- Vol. 46 (4) , 275-283
- https://doi.org/10.3168/jds.s0022-0302(63)89032-3
Abstract
The purified milk lipase gave a typical protein UV absorption spectrum with maximum absorption at 277 m[mu] and minimum at 251 m[mu], with A277/A251 ratio being 2.34. The enzyme contained 49.63% C, 7.51% H2, 14.33% N, 1.04% S, and 0.26% P. The purified enzyme is distinctly different in its characteristics from other known milk proteins and enzymes. It probably exists as a physically absorbed unit on casein particles.This publication has 16 references indexed in Scilit:
- Methodological aspects of gel filtration with special reference to desalting operationsJournal of Chromatography A, 1961
- Pancreatic LipasePublished by Wiley ,1961
- Purification of hog pancreatic lipaseArchives of Biochemistry and Biophysics, 1959
- Zone Electrophoresis in Starch Gels and its Application to Studies of Serum ProteinsAdvances in Protein Chemistry, 1959
- The Minor Proteins of Bovine MilkJournal of Dairy Science, 1958
- On the Nature of Milk Lipase(S)Journal of Dairy Science, 1957
- A Rapid Silica Gel Method for Measuring Total Free Fatty Acids in MilkJournal of Dairy Science, 1956
- Milk Lipase. I. The Lipolytic Activity of Separator SlimeJournal of Dairy Science, 1955
- Determination of Nitrogen in Biological MaterialsAnalytical Chemistry, 1948
- Effect of Certain Factors upon Lipolysis in Homogenized Raw Milk and CreamJournal of Dairy Science, 1941