Mammalian Cell-Virus Relationship. III. Poliovirus Production by Non-primate Cells Exposed to Poliovirus Ribonucleic Acid.

Publisher Website
Google Scholar
Abstract
Naturally insusceptible non-primate cells, after a latent period following exposure to poliovirus RNA, in vitro or in vivo produced infectious poliovirus. RNA activity was destroyed by ribo- nuclease treatment. RNA-exposed non-primate cells in culture accumulated infectious virus intracellularly before release. By use of viral RNA, it was possible to bypass specific receptor mechanisms of the cells to infect both primate and non-primate cells to about the same extent. Extracellular release of infectious poliovirus by non-primate cells lacking specific receptors suggests that such receptors are not essential for poliovirus release. Present experience indicates that infectious poliovirus produced by RNA-exposed insusceptible cells and animals is indistinguishable from the virus donating the RNA; progeny virus has been found readily neutralized by homotypic antiviral serum, unaffected by ribonuclease, and unable to infect such non-primate cells as produced it.

This publication has 5 references indexed in Scilit: