Reverse transcriptase from avian myeloblastosis virus

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Abstract
From lots of 20-30 g of avian myeloblastosis virus RNA-dependent DNA polymerase was obtained in preparations of purity greater than 95% by using a 2-step column chromatographic procedure employing DEAE (DE 52) and carboxymethylcellulose (CM 52). Yields of RNA-dependent DNA polymerase varied from about 20,000-35,000 U/g of virus. Specific activity of the enzyme was about 35,000-60,000 U/mg of protein. Free of detectable RNase activity, the product exhibited a MW of about 160,000, an isoelectric point of 6.5 and about 2 mol of fatty acid per mol of enzyme.