Synthesis of full-length DNA copies of avian myeloblastosis virus RNA in high yields.
- 1 July 1977
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 74 (7) , 2840-2843
- https://doi.org/10.1073/pnas.74.7.2840
Abstract
The synthesis of full-length complementary[c] DNA copies of avian myeloblastosis virus RNA [using reverse transcriptase] is described. The cDNA is estimated by electrophoresis in agarose-methylmercury gels to have a MW of 2.6 .times. 106, equivalent in size to that of the RNA template. Most (60-70%) of the reaction product is the full-length material and yields correspond to 60-70% of the input RNA.Keywords
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