SODIUM ARACHIDONATE INDUCED INVITRO POLYMORPHONUCLEAR LEUKOCYTE AGGREGATION

  • 1 January 1984
    • journal article
    • research article
    • Vol. 43  (3) , 393-405
Abstract
Human polymorphonuclear leukocytes (PMN) aggregated to sodium arachidonate (AA) in a dose depedent manner. Other agents such as epinephrine or ADP were unable to initiate aggregation. The cells released lactate dehydrogenase (LDH) during aggregation, and EM demonstrated distinct morphological changes in PMN aggregated with AA. Indomethacin enhanced AA induced aggregation, whereas 1-methylimidazole inhibited aggregation and LDH release. Preincubation with cytochalasin B (CTB) did not enhance AA induced aggregation, nor did it reduce LDH release. The effects of indomethacin or 1-methylimidazole on AA induced aggregation were unaltered by the presence of CTB. The results indicate that LDH release occurs as a function of the aggregation process rather than a non-specific effect; competition between cyclooxygenase and lipoxygenase occurs; and shifting this competition in favor of lipoxygenase enhances the PMN aggregation process.