Dependence of cytoplasmic on mitochondrial protein synthesis inK. lactis CBS 2360
- 1 January 1977
- journal article
- research article
- Published by Springer Nature in Molecular Genetics and Genomics
- Vol. 150 (2) , 137-140
- https://doi.org/10.1007/bf00695393
Abstract
In the “petite negative” yeastKluyveromyces lactis (strain CBS 2360) growth is inhibited by the same Erythromycin concentrations on fermentable and non-fermentable carbon sources. The minimal inhibitory concentration of Erythromycin does not affect respiration, but completely inhibits total aminoacid incorporation by the cells in conditions in which permeability effects can be ruled out. The effect of the antibiotic on the “in vitro” protein synthesis by cytoplasmic and mitochondrial systems shows that inhibition is effective only at the mitochondrial level. Results suggest that the inhibition of growth and of total protein synthesis is a consequence of the inhibition of mitochondrial protein synthesis.This publication has 12 references indexed in Scilit:
- Reversible and permanent effects of the carbon sources and various antibiotics on the morphology and metabolic properties of Ustilago cynodontis cells.The Journal of cell biology, 1975
- Promitochondria of Anaerobically Grown YeastJournal of Biological Chemistry, 1972
- Erythromycin inhibition of sporulation inSaccharomyces cerevisiaeCellular and Molecular Life Sciences, 1971
- Role of the mitochondrion in the regulation of protein synthesis in the eucaryote Saccharomyces cerevisiaeMolecular Genetics and Genomics, 1971
- Synthesis of mitochondrial and nuclear DNA in anerobically grown yeast during the development of mitochondrial function in response to oxygenJournal of Molecular Biology, 1969
- Invivo differentiation of yeast cytoplasmic and mitochondrial protein synthesis with antibioticsBiochemical and Biophysical Research Communications, 1966
- Site of action of cycloheximide in cells of Saccharomyces pastorianusBiochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis, 1965
- Amino Acid Incorporation into Protein by Cell-free Extracts of Yeast*Biochemistry, 1963
- Large-scale preparation of yeast “soluble” ribonucleic acidBiochemical and Biophysical Research Communications, 1963
- PROTEIN MEASUREMENT WITH THE FOLIN PHENOL REAGENTJournal of Biological Chemistry, 1951